Publications
Learn about the practices followed by University of Copenhagen and what University of Stanford and University of Berlin are saying about our kits in their upcoming publications. Request a publication

  • Title: A Simple Method for Validation and Verification of Pipettes Mounted on Automated Liquid Handlers

    Michael Stangegaard, Anders J. Hansen, Tobias G. Froslev, and Niels Morling Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark (JALA 2011;16:381-6)

    Description:
    We have implemented a simple, inexpensive, and fast procedure for validation and verification of the performance of pipettes mounted on automated liquid handlers (ALHs) as necessary for laboratories accredited under ISO 17025. A six- or seven-step serial dilution of OrangeG was prepared in quadruplicates in a flat-bottom 96-well microtiter plate, manually using calibrated pipettes. Each pipette of the liquid handler (1e8) dispensed a selected volume (1e200 mL) of OrangeG eight times into the wells of the microtiter plate. All wells contained a total of 200 mL liquid. The absorbance was read, and the dispensed volume of each pipette was calculated based on a plot of volume and absorbance of a known set of OrangeG dilutions. Finally, the percent inaccuracy (%d) and the imprecision (%CV) of each pipette were calculated. Using predefined acceptance criteria, each pipette was then either approved or failed. Failed pipettes were either repaired or the volume deviation was compensated for by applying a calibration curve in the liquid-handler software. We have implemented the procedure on a Sias Xantus, an MWGt The ONYX, four Tecan Freedom EVO, a Biomek NX Span-8, and four Biomek 3000 robots, and the methods are freely available. In conclusion, we have set up a simple, inexpensive, and fast solution for the continuous validation of ALHs used for accredited work according to the ISO 17025 standard. The method is easy to use for aqueous solutions but requires a spectrophotometer that can read microtiter plates.

    Posters
    Learn about some of the work we did with our users present as posters at the Society for Laboratory Automation and Screening (SLAS.org)

  • Title: User Happy Hour -- Enabling Enabling Labs to Maximize the Performance of Their Robots

    Presented at SLAS2012

    Description:
    "You automated what?" "Liquid handling of?" The automation of liquid handling has always been a challenge and a dream of many based on the application, level of automation, the scale, speed, and the amount. Automated liquid handling equipment is an increasingly essential component of life science laboratories with a growing need to more effectively and conveniently process samples (refer to OpenBiobank.com talk and poster). Used to boost the productivity and repeatability of volume transfers, automated liquid handlers must be optimized to achieve precision and accuracy in liquid delivery. To address this need, Automation Trainer (www.automationtrainer.com) a leading expert in laboratory automation and training has partnered with the user community and lab automation equipment vendors to provide a unique approach to help the user community understand liquid handling and thereby enable Labs to Maximize the Performance of their Robots and their resources. Our users have expressed their challenges and needs for automating but more their desire to understand the process of liquid handling. We will discuss our user communities various liquid handling applications, the approaches, and their treatment of lab automation. We will also present a poster which summarizes our talk. Poster- Our users have expressed their challenges and needs for automating but more their desire to understand the process of liquid handling. The automation of liquid handling has always been a challenge and a dream of many based on the application, level of automation, the scale, speed, and the amount. We will discuss our user communities various liquid handling applications, the approaches, and their treatment of lab automation. We will also present a poster which summarizes our talk. 40 word Summary- Users expressed their challenges/needs for automating to understand the process of liquid handling. Liquid handling automation has always been a challenge and a dream of many based on the application, level of automation, the scale, speed, and the amount.

  • Presented at SLAS2012

    Description:
    Compelling innovation-- ALH equipment is an increasingly essential component of life science laboratories. Used to boost the productivity, repeatability of volume transfers, ALH must be optimized to achieve precision and accuracy in liquid delivery. To address this need, AT, is a leading expert in laboratory automation and training, and a supplier of liquid handling quality assurance measurement systems for calibrating equipment. Based on years of practical laboratory experience teaching thousands of students, we have developed a comprehensive curriculum for ALH instruction captured in a single dye calibration kit meeting industry demand and user pains. Significant scientific or market need-- Our users are sales, service, and researchers that all utilize ALHs that require calibration with actual CVs that measure accuracy and precision: sales needs to prove instrument capabilities convincing customers with demonstrations that perform actual pipetting within specifications and require minimal setup time, service people need an efficient and cost effective method for calibrating equipment in the field for site or factory acceptance. Users need optimum performance out of their equipment to not experience process cost impacts and validate processes for QA/QC. A single dye calibration kit is needed to assure users of proper functionality after every service call, install, move, and operation to qualify demonstrations and operational requirements. Market opportunity-- Is to satisfy the user driven market need of a single dye non-NIST traceable calibration kit from educationally developed good practices adopted from trustworthy relationships with users and vendors and long withstanding hands on trainings based on years of industry experience dedicated to our students. We have developed a user community through our educational course and can satisfy their pain of having an easy, trustworthy, reliable, time efficient, and cost effective method for calibrating their equipment for their specific instrument, liquid class, and volume range.

  • A QC and Calibration Kit for Determining Reproducible results

    Presented at SLAS2014

    Description:
    Automated Liquid Handling (ALH) equipment is an increasingly essential component of life science laboratories. Used to boost the productivity, repeatability of volume transfers, ALH must be optimized to achieve precision and accuracy in liquid delivery. To address this need, AT (Automation Trainer LLC), is a leading expert in laboratory automation and training, and a supplier of liquid handling quality assurance measurement systems for calibrating equipment. Based on years of practical laboratory experience teaching thousands of students, we have developed a comprehensive curriculum for ALH instruction captured in a single dye calibration kit meeting industry demand and user pains. Significant scientific or market need-- Our users are sales, service, and researchers that all utilize ALHs that require calibration with actual CVs that measure accuracy and precision: sales needs to prove instrument capabilities convincing customers with demonstrations that perform actual pipetting within specifications and require minimal setup time, service people need an efficient and cost effective method for calibrating equipment in the field for site or factory acceptance. Users need optimum performance out of their equipment to not experience process cost impacts and validate processes for QA/QC. A single dye calibration kit is needed to assure users of proper functionality after every service call, install, move, and operation to qualify demonstrations and operational requirements. Market opportunity-- Is to satisfy the user driven market need of a single dye non-NIST traceable calibration kit from educationally developed good practices adopted from trustworthy relationships with users and vendors and long withstanding hands on trainings based on years of industry experience dedicated to our students. We have developed a user community through our educational course and can satisfy their pain of having an easy, trustworthy, reliable, time efficient, and cost effective method for calibrating their equipment for their specific instrument, liquid class, and volume range.

  • Biospecimen Exchange, Biotech cloud, and Biobank Registry: Biolocator of Biobanks, Biomarker sites, Collection Sites, and Validated Results

    Presented at SLAS2014

    Description:
    Compelling innovation OBB (Biotechcloud.org, Openbiobank.org, biospecimenexchange.org) provide real time access to quality biospecimen and attempt to close the current gap on the common problem in scientific human disease research of having inadequate supply of quality samples and data preventing quality research results and inhibiting personalized medicine. OBB challenges the current industry dropout rate figures of 99% by attempting to guarantee quality for end users. It achieves this through a process of reputable and trustworthy relationships with suppliers and consumers, partnerships, and collaborators with research organizations and biobanks as well as application of best practices from employing standards, technology, and education. This is through the bio-locator "Biobankregistry.org" which registers all specimen types (DNA,RNA, protein, biofluids, and tissue) and puts the types of biobanks on the world map publishing to the community and recognizing their value to the community. Biomarkers are classified on the biospecimen exchange with associated specimen. Data is easly uploaded via the biotechcloud.org which is a drag and drop drive. One may select to share selectable groups and provide feedback on various specimen data. Users exchange specimen and data via an "Amazon-like" web site biospecimenexchane.org. Its online system fosters collaboration among biobanks around the world with a common interest in combating disease and sources quality samples and data conveniently to researchers making it easy to find specific samples and fulfill requests for rare biospecimen. We shall discuss Its state of the art services that are business and user community focused and its continual service improvement feedback model from end users and suppliers. In addition, OBB attempts to minimize liability for suppliers and consumers with industry standards that are community driven and government enforced. Significant scientific or market need according to the Director of NCI, current cancer research is plagued with “garbage in” samples producing “garbage out” results and publication pollution. Brokered biospecimen supplies have a 99% fall out rate which is due to improper sample handling and inadequate distribution producing “poor quality” results and wasting precious research resources. Researchers cannot conveniently search and obtain quality biospecimen. Personalized medicine research depends on having an adequate supply of high quality biospecimen AND real time access to these samples. We envision providing researchers with onestop shop for biospecimen and clinical data acquisition. We shall present the automated tissue broker model, effective and efficient sourcing and acquisition of samples, its importance to laboratory automation, classification of biomarkers and data. We shall demonstrate search options that automate the biospecimen broker business and how it facilitates research conducted by our clients and help biospecimen suppliers to better serve the researchers’ needs for obtaining quality samples that will serve as reliable input to laboratory automated processes and sample preparation for laboratory experiments.

  • Inexpensive Standardized QC and Calibration Kit for Determining Reproducible Results

    SLAS2014, Dr. Martin NeuenSchwander FMP Institute University of Berlin, Dr. John Coller Stanford University Worked performed at University of Berlin, Stanford, Galapagos, and Genetech

    Description:
    Automated Liquid Handling (ALH) equipment is an essential component of laboratories. Used to boost the productivity, repeatability of volume transfers, ALH must be optimized to achieve precision and accuracy in liquid delivery. To address this need, AT (Automation Trainer LLC), is a leading expert in laboratory automation and training, and a supplier of liquid handling quality assurance measurement systems for calibrating equipment. Based on years of practical laboratory experience teaching thousands of students, we have developed a comprehensive curriculum for ALH instruction captured in a single dye QC calibration kit meeting industry demand and end user pains. Users need optimum performance out of their equipment to not experience process cost impacts and to validate processes for QA/QC. A single dye calibration kit is needed to assure users of proper functionality after every ser-vice call, install, move, and operation to qualify demonstrations and operational requirements. For Stanford University, FMP, and Galapagos we satisfied the need of a single dye non- NIST traceable calibration kit from educationally developed good/best practices adopted from our trustworthy relationships with users and vendors and long withstanding hands on trainings based on years of industry experience dedicated to our students. We have developed a user community through our educational course and can satisfy their pain of having an easy, trustworthy, reliable, time efficient, and cost effective method for calibrating their equipment for their specific instrument, liquid class, and volume range.
 
Our Books
  • Title: Microarrays and BioChip Technology

    By Douglas Gurevitch

    Description:
    Microarrays promise to speed up genetic analysis much the same as microprocessors speed up computation. Biochips are used to examine gene activity and identify gene mutations using a hybridization reaction between sequences on the microarray and a fluorescent sample.

  • Title: Laboratory Automation and LIMS in Forensics

    M Stangegaard, AJ Hansen, and N Morling, University of Copenhagen, Copenhagen, Denmark

    Description:
    Cited from our Good Liquid Handling chapter the Good Liquid Handling kit is a method to achieve easy, simple, robust, reliable, dependable, volume verification in a box. You can order the entire book chapter by requesting access.

 
White Papers
  • Title: Fixed Tip and Reused Disposable Tip Washing Validation

    Douglas Gurevitch and Petar Stojadinovic
    Former Senior Systems Engineers formerly with Sequana Therapeutics

    Description:
    In 1995 an effort to increase the use of liquid handling automation was undertaken at Sequana Therapeutics as a strategic initiative to increase throughput to enable the genotyping goals of the company. As part of this initiative, the company was interested in investigating carry-over contamination on fixed tip systems versus using disposable tips for each pipetting liquid transfer. The obvious cost savings were to be immense given the projected throughput of the experimental plan. The processes to be setup included liquid handling to set up Polymerase Chain Reaction (PCR) amplifications as part of the genotyping work. Later, this was extended to investigate re-using pipette tips by washing them between uses for cost savings when using machines that only had disposable tip pipetting.

  • Title: Solvent Compatibility

    Description:
    A look at various solvent compatibility options and discussions on what and how to replace components if affected.

  • Title: Common Service Issues

    Description:
    A discussion of various symptomatic service issues, how to avoid and treat.

  • Title: Gravimetric Protocol for calibration

    Description:
    A simple protocol for gravimetric calibration.

  • Title: Introduction to BioPrint

    Description:
    The goal of the BioPrint™ consortium is to generate models that correlate molecular features of a compound to patterns of in vitro and in vivo biological activity. In order to accomplish this goal we have screened a large number of reference compounds against a large panel of pharmacology and pharmaceutical assays. This data is used to generate a BioPrint™ for each compound. The BioPrint™ is a unique fingerprint that represents the molecular, in vitro and in vivo features of a particular compound. A variety of data mining techniques are being used to analyze the BioPrints in order to generate structure-activity relationship (SAR) models. However, unlike traditional SAR techniques the BioPrint approach emphases non-linear techniques such as statistical clustering and recursive partitioning in order to capture loose, nonlinear correlation between pharmacophoric and other calculated molecular descriptors and the biological properties of compounds.

  • Title: Disposable versus Fixed Tips

    Description:
    Discussion about when to use disposable versus fixed tips

  • Title: Disposable versus Fixed Tips

    Description:
    Discussion about when to use disposable versus fixed tips

  • Title: How to select appropriate Liquid Class Settings

    Description:
    Understanding what liquid classes are requires knowledge is selecting the appropriate dispense and aspiration settings and following a good automated pipette technique. We discuss the methods of selection and the results in selecting various settings.

  • Title: Conducting IQ/OQ Service qualifications

    Description:
    We discuss the proper method in conducting instrument installation qualification and operational qualification and when needed to conduct them.

  • Title: "Best Practices" for a Biospecimen

    Resource for the Genomic and Proteomic Era
    Prepared for the National Cancer Institute as a National Dialogue on Cancer

    Description:
    Human tissue has been collected and stored at institutions in the United States for more than 100 years. Each of these institutions, or repositories, was established to meet a specific set of objectives, and its design is integrally linked to those objectives. Tissue collection, processing, and storage techniques vary depending on the purpose of the repository, as do the quality and extent of information collected with the biospecimens.

  • Title: "Best Practices" for a Biospecimen

    Resource for the Genomic and Proteomic Era
    Prepared for the National Cancer Institute as a National Dialogue on Cancer

    Description:
    Human tissue has been collected and stored at institutions in the United States for more than 100 years. Each of these institutions, or repositories, was established to meet a specific set of objectives, and its design is integrally linked to those objectives. Tissue collection, processing, and storage techniques vary depending on the purpose of the repository, as do the quality and extent of information collected with the biospecimens.

  • Title: Good Sample collection and sample studies

    Description:
    We discuss the methods in conducting good sample collection and good liquid handling methods that can produce reproducible results in conducting sample studies.

  • Title: Quality Results through Volume Verification

    Description:
    In this presentation we discuss the process of volume verification.

  • Title: Economic Justification

    Description:
    In this presentation part of a semester course presented at UCSD we discuss all the financial components part of Lab Automation in justifying equipment purchases. We discuss what to research in acquiring Automated Liquid Handling equipment acting as a “Buyer’s Guide” in purchasing Good Liquid Handling equipment and accessories. Case study report includes an examination of a real process internal to your work environment where you do a comparison to aid in a purchasing/planning decision.

  • Title: AGILE BUSINESS ANALYSIS FOR LAB AUTOMATION

    Description:
    Business analysis is the discipline used to pinpoint answers to these questions and build the right automation requirements. But the last thing good software developers want or need is a long list of requirements. Instead of text-based requirements, show them what you want by getting to visual. This means including Agile business analysis as part of your strategy. Agile business analysis includes only the artifacts you need as well as the methods used to design and build or customize automation -so you can get it right the first time. Whether you’re building an e-notebook, liquid-handling system, or automating any element of your lab, business analysis offers a powerful set of methods and artifacts that will get you there faster and smarter.

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